General Cell Based Assays
Indigo Biosciences Human Insulin-Like Growth Factor Receptor 1 (IGF-1R) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Insulin-Like Growth Factor Receptor 1 reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against Insulin-Like Growth Factor Receptor 1. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request).
Indigo Biosciences Human Insulin Receptor isoform B (INSRb) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Insulin Receptor, isoform B reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against Insulin Receptor, isoform B. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor (GM-CSFR, CSF2R) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against GM-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results
Indigo Biosciences Human Adrenoceptor Beta 1 (ADRB1) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Beta 1 reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRB1. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Dojindo Molecular Technologies Inc Mito-FerroGreen (1 set 50UG x2) is a novel fluorescent probe for the detection of ferrous ions (Fe2+) in mitochondria, used in ferroptosis research.
Iron is the most abundant transition metal element within an organism and exhibits various physiological activities. Free iron in living cells is particularly important because of its high reactivity, suggesting a relationship to cellular damage or death. Free iron exists in its stable redox states, ferrous ion (Fe2+) and ferric ion (Fe3+). In living cells, research involving the behavior of Fe2+ is necessary to understand the intracellular reductive environment, metal transporters and water solubility of Fe2+. Mito-FerroGreen is a novel fluorescent probe for the detection of ferrous ion (Fe2+) in mitochondria where Fe-S clusters and heme proteins are synthesized, and enables live cell fluorescent imaging of intracellular Fe2+.Mito-FerroGreen is widely used in ferroptosis research, one of the various research fields Dojindo has a strong portfolio in. (https://www.dojindo.com/ferroptosis-detection-selection-guide/)
Sartorius iQue Screener Plus Validation Beads
Perform daily quality control maintenance using iQue Screener Plus Validation Beads – easy to use and economic with two simple drops in 300 uL Qsol or Buffer.
Dojindo Molecular Technologies Inc Mitophagy Detection Kit (1 set, 5UG x1) allows for mitochondria research for mammalian cells. No need for transfection, simply add Mtphagy Dye!
Mitochondria plays a crucial role in cells such as production of energy for cell viability. Mitophagy was recently found to be related to Alzheimer and Parkinson disease induced by the accumulation of depolarized mitochondria. Mitophagy serves as a specific elimination system that dysfunctional mitochondria caused by oxidative stress and DNA damage are sequestered into autophagosome, fused to lysosome and degraded by digestion. This kit is composed of Mtphagy Dye (reagent for detection of mitophagy) and Lyso Dye. Mtphagy Dye accumulates in intact mitochondria, is immobilized on it with chemical bonds and exhibits a weak fluorescence from the influence of surrounding conditions. When Mitophagy is induced, the damaged mitochondria fuses to lysosomes and then Mtphagy Dye emits a high fluorescence. To confirm the fusion of Mtphagy Dye labeled mitochondria and lysosomes, Lyso Dye included in this kit can be used.
Abcam Wound Healing Assay
Wound Healing Assay
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CCR2 Total GPCR Internalization Assay, 10pl Kit. (93-0679E3CP5L)
The PathHunter CCR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CCR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc Phagocytosis Assay, Zymosan Substrate, 96 assays
CytoSelect™ 96-Well Phagocytosis Assay, Zymosan Substrate
Meso Scale Discovery R-PLEX Human HSP27/HspB1 ASY
The R-PLEX Human HSP27 (HSPB1) Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that measures human HSP27 protein levels in serum plasma cell culture supernatants cell and tissue lysates and other biological samples This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker solutions calibrator and controls This assay allows for the quantitative measurement of HSP27 levels with high precision and reproducibility
Meso Scale Discovery U-PLEX Mouse TNF-RI ASY
The Meso Scale Discovery U-PLEX Mouse TNF RI Assay Kit is a multiplex immunoassay that measures mouse tumor necrosis factor receptor 1 (TNFR1) in serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and buffer for sample dilution It can accurately quantify TNFR1 levels with minimal sample volume requirements This kit is for research use only